SubtiBank SubtiBank
yetO [2019-06-25 08:41:16]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yetO [2019-06-25 08:41:16]

cytochrome P450 (CYP102A2)/ NADPH-cytochrome P450 reductase
Locus
BSU07250
Isoelectric point
5.73
Molecular weight
119.26 kDa
Protein length
1061 aa Sequence Blast
Gene length
3186 bp Sequence Blast
Function
fatty acid metabolism
Product
NADPH-cytochrome P450 reductase
Essential
no
E.C.
1.6.2.4
Synonyms
yfnJ

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
792,682 795,867

The protein

Catalyzed reaction/ biological activity

  • oxidation of even- and odd-chain saturated and unsaturated fatty acids to the respective omega-3, omega-2 and omega-1 hydroxylated fatty acids PubMed
  • Paralogous protein(s)

    Cofactors

  • FAD (according to UniProt)
  • Structure

  • 2X7Y (from Bacillus megaterium; 66% identity, 87% similarity) PubMed
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • MGNA-C225 (yfnJ::erm), available at the NBRP B. subtilis, Japan
  • BKE07250 (yetO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGAACCATTCCTTTCCG, downstream forward: _UP4_TAGATAAAGAAGACTGGAGA
  • BKK07250 (yetO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGAACCATTCCTTTCCG, downstream forward: _UP4_TAGATAAAGAAGACTGGAGA
  • References

    Loading